Optimal concentration of modified platelet-rich plasma to promote the proliferation of dental pulp stem cells from deciduous teeth

doi:10.3969/j.issn.2095-4344.2014.28.015

Abstract

Abstract:

BACKGROUND: Previous experiments have shown that modified platelet-rich plasma activated by liquid nitrogen freezing and thawing can promote the proliferation of human bone marrow mesenchymal stem cells and dental pulp stem cells in a concentration-dependent manner.
OBJECTIVE: To investigate the effect of modified platelet-rich plasma at different concentrations on the proliferation of dental pulp stem cells from human exfoliated deciduous teeth.
METHODS: Platelets were selected and harvested by automatic blood cell analyzer, and then activated by liquid nitrogen freezing and thawing. α-MEM served as basal medium. Different concentrations of modified platelet-rich plasma (2%, 5%, 10%, 20%) or 10% fetal bovine serum were added, respectively. The difference in cell proliferation was observed.
RESULTS AND CONCLUSION: Modified platelet-rich plasma at different concentrations could promote the proliferation of dental pulp stem cells from deciduous teeth. The effects of 2% modified platelet-rich plasma and 10% fetal bovine serum on promoting the proliferation of dental pulp stem cells from deciduous teeth were similar. These results indicated that 2% modified platelet-rich plasma could promote the proliferation of dental pulp stem cells from deciduous teeth, and substitute for fetal bovine serum in the amplification of dental pulp stem cells from deciduous teeth in vitro.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: platelet-rich plasma, tooth, deciduous, dental pulp, serum albumin, bovine

CLC Number:

R394.2

Wen Jun, Wu Bu-ling, Duan Jian-min, Li Hong-tao, Li Si-han, Li Xin. Optimal concentration of modified platelet-rich plasma to promote the proliferation of dental pulp stem cells from deciduous teeth[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(28): 4517-4523.

Figures/Tables 6

2.4 改良富血小板血浆对乳牙牙髓干细胞生长的影响表1所示为不同浓度改良富血小板血浆培养的乳牙牙髓干细胞在不同时间段的增殖活性。不同培养条件下乳牙牙髓干细胞的生长曲线见图5，随着时间的延长，各组细胞数量都增加，说明各浓度的改良富血小板血浆都能够促进乳牙牙髓干细胞的增殖。实验第2天，体积分数2%改良富血小板血浆对乳牙牙髓干细胞的促增殖效果与对照组差异无显著性意义(P > 0.05)，而5%，10%和20%改良富血小板血浆组无论与对照组或2%改良富血小板血浆组比，促增殖效果较弱(P < 0.05)；体积分数5%，10%和20%改良富血小板血浆的促增殖效果相当(P > 0.05)。实验第5天，体积分数2%和5%改良富血小板血浆对乳牙牙髓干细胞的促增殖效果与对照组差异无显著性意义 (P > 0.05)，与对照组和2%改良富血小板血浆组相比，10%和20%改良富血小板血浆促乳牙牙髓干细胞的增殖效果较弱，但差异有显著性意义(P < 0.05)，但是与5%改良富血小板血浆组相比，10%和20%改良富血小板血浆组促乳牙牙髓干细胞的增殖能力的差异无显著性意义(P > 0.05)。实验第7天，与对照组相比，体积分数2%和5%改良富血小板血浆对乳牙牙髓干细胞的促增殖效果差异无显著性意义(P > 0.05)，但与对照组、2%和5%改良富血小板血浆组相比，体积分数10%和20%改良富血小板血浆具有促增殖效果(P < 0.05)。体积分数2%改良富血小板血浆在整个培养周期中与体积分数10% 胎牛血清对乳牙牙髓干细胞的促增殖效果差异无显著性意义(P > 0.05)。各浓度富血小板血浆相比较，体积分数2%改良富血小板血浆对细胞的促增殖能力最强；与体积分数10%胎牛血清比较，体积分数5%改良富血小板血浆在第2至4天对细胞的促增殖能力较弱，但是在第5-7天，两者比较差异无显著性意义(P > 0.05)；与体积分数10%胎牛血清相比，体积分数10%和20% 改良富血小板血浆在整个细胞培养周期内对细胞的促增殖能力较弱(P < 0.05)。 "

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